Page 3: Safety Instructions Safety Instructions This instrument may sometimes be used with hazardous materials, operations, and equipment. It is beyond the scope of this manual to address all of the potential safety risks associated with its use in such applica- tions. It is the responsibility of the user of this instrument to consult and establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use. Page 4: Functions And Limitations Of Use Functions and Limitations of Use Limitations of Use Operating Limitations This instrument is designed for dispensing liquids, Liquids, which form deposits may accelerate wear observing the following physical limits: on the piston seal, and make the piston difficult to move or may cause jamming e.

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The notable exceptions are: NaN3 sodium azide : Found in many biology labs, and used as a preservative for buffers. It also attacks these materials in almost any other bottle-top dispenser using these materials. Because sodium azide is often used in solution with other chemicals, confirm the compatibility of all components of the liquid before use.

Physical Limitations: Any liquid that has a density of over 2. The major consideration for hot applications is that liquids dispensed at higher temperatures, like agar, usually are done so because the liquid becomes less viscous at these temperatures. A customer may try these temperatures, but at their own risk, as it will void the warranty. Vapor pressure over mbar torr or extremely volatile liquids.

Liquids that have a high vapor pressure tend to vaporize inside the dispenser, causing liquid to flow out of the dispenser even when the instrument is not being operated.

A common volatile liquid is Acetone aka "Nail Polish Remover" which has a vapor pressure of mbar at room temperature, and will work fine. The Dispenser selection chart listing many commonly used laboratory liquids can be found in the BrandTech catalog, or on our website: click here. This optimizes it for use with concentrated acids and organic solvents that can interact with the PFA, but makes it less useful for bases. Cleaning instructions are located in the manual. If you do not have a copy, click here for the electronic version.

What is happening? Check to ensure they are attached properly, and that the sealing washers are in place. The filling valve is most often loose because the autoclaving instructions were not followed closely.

Recheck the connections. Clean the valve. What should I do? Good places to look are at the junction of the filling tube and filling valve. If it does not make a tight seal, trim some of the tube off, and reattach.

Check to see if the filling and discharge valves are tight, and the sealing washers are in place. Lastly, is there any liquid in the bottle? How do I stop this? This directs liquid down the side of the bottle, to help keep from generating additional bubbles when priming. When absent, some liquid can dribble down the side of the instrument. If you need a replacement, the part number is and can be ordered from your favorite supplier. Crystals have probably gotten between your piston and cylinder, and are grinding away the piston and seal.

If the instrument has been operated in this condition, it is possible that the piston and cylinder are scored. Gently remove the piston, and follow the cleaning procedure as described in the manual. Retry the dispenser. Continued use of the dispenser in this condition can destroy it.

I broke my piston or cylinder. Can I purchase a replacement? This is so that the instrument can have smooth one-handed operation without the need for a seal. However, this makes piston replacement impossible. If you break either your piston or cylinder, call Customer Service, and ask about our Exchange Program. By mounting the instrument directly onto the reagent bottle, poured transfer, and associated spilling, is eliminated. Additionally, the likelihood that we would be able to guess your preferred bottle is extremely small.

What size adapter do I need for this bottle? Most 1 liter and 1 quart bottles in the United States have a 33mm thread. Most 4 liter or 1 gallon bottles in the US have a 38mm thread, as do many scintillation fluid bottles. Page 51 specifically refers to Autoclaving. Note: While both gravity and pre-vac are acceptable methods, pre-vac is recommended. Either setting is fine, as long as the other parameters of time and temperature are met.

In general, pre-vac ensures a greater assurance of sterility. If the instrument is not given sufficient drying time in the autoclave, some condensation may collect as droplets just below the housing, on top of the valve block. It should NOT be installed for general purpose dispensing, as it has limited chemical resistance. Complete instructions are in the manual. If you do not have a copy of the manual, click here for the electronic version.

There are currently several different sterilizers on the market using hydrogen peroxide, either vaporized or as a gas plasma e. As such, it will void the warranty. What standard does the recommended steam sterilization autoclaving procedure reference? In addition to autoclaving the dispenser, you may want to consider the use of our micro-filter connector, catalog number This filters the air coming inside the bottle to displace the liquid, to keep out bacteria.

Is this the performance for my specific instrument? They are not provided with individual calibration certificates. Are there any precautions I should take when dispensing scintillation fluid? Many scintillation fluids precipitate in the presence of water. You should make sure the instrument is completely dry before use. Additionally, in humid climates, it may be necessary to use a drying tube to keep atmospheric moisture from contaminating your scintillation fluid.

What is the function of the drying tube? When a dispenser dispenses liquid, air needs to enter the container to displace the liquid. If the liquid is sensitive to components in the air, for example, water vapor, a drying tube is recommended, so that an appropriate drying agent can remove the water.

The quantities of drying reagent that we could possibly sell in a year would not be enough to assure that our customers would get fresh reagent at a reasonable price. Drierite is a popular drying reagent available from most laboratory supply dealers. A longer filling tube may be required. As each circumstance is different, additional parts may be required. Is a discharge tube available with a Luer Lock fitting? A discharge tube with a Luer Lock fitting for the attachment of filters can be ordered as catalog number This discharge tube is not suitable for peroxides.

Care must be taken while dispensing not to create excess back pressure. Can I use a flexible discharge tube with my 0. Yes, and no. The 1 - 10mL flexible discharge tube catalog number will fit the 0. However, because of the length of the tubing, dispensing of volumes below 2mL will not meet the accuracy and precision specifications that BRAND publishes.

Depending on the application, this may be fine for the user. This also applies to volumes below 2mL on the 0. What thread adapter fits my CareFusion saline bottle? CareFusion saline bottles have a 35mm thread. The thread adapter to fit this bottle is catalog number The discharge tube drips, my assays are inaccurate, and when left for a while, the piston begins to rise all by itself.

The hydrogen peroxide breaks down into water and oxygen gas. The gas is what is forcing out the liquid, and pushes the piston up. Essentially, the customer is using water for their assay. What is accuracy, and what is precision? Accuracy is how close to a desired target value a test result is. Precision is how close a series of test results are to each other. What do "calibration", "gravimetric testing", and "adjustment" mean? Calibration actually refers to the act of measuring results from an instrument, and determining its accuracy and precision.

Gravimetric testing is one method of performing calibration. In the case of liquid handling instruments, it usually means the repeated weighing of dispensed amounts of distilled water water free of impurities.

Distilled water has a defined density at specific temperature and atmospheric pressure. By knowing the mass of water dispensed, it is possible to know the actual volume dispensed, and from that, determine the accuracy and precision of the instrument. Adjustment is the physical changing of the instrument that was calibrated. This can be in two ways. The conventional way is to change the volume dispensed to represent more closely the display. This requires at least two, usually more calibrations.

One to check the instrument, another to check the instrument after adjustment, readjustment of the instrument if the first correction was done improperly, and so on.


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