Mezilar Catalyze hydrolysis of cles, a protein nucleus with commerciall positive surface charge is a-1,4-glycosidic linkages in pectic acid polygalacturonic coated by negatively charged pectin molecules Pilnik acid. Reproduction and synthesis of pectolytic enzymes by Clostridium Immobilization of pectinesterase on c-alumina for the treatment of felsincum on media with various carbon sources. Applications of pectinases in the commercial sector: a review. Purification and characterization of pectolytic enzymes on by virulent and hypovirulent isolates of Rhizoctonia solani KUHN. These residual of the pulp wash. Maceration in relation to the post handling and processing of plant material.

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Kashyap a, P. Vohra a, S. Chopra a, R. These enzymes break down complex polysaccharides of plant tissues into simpler molecules like galacturonic acids. The role of acidic pectinases in bringing down the cloudiness and bitterness of fruit juices is well established.

This review discusses various types of pectinases and their applications in the commercial sector. All rights reserved. Keywords: Pectinase; Industrial applications 1. Introduction 2. The side chains juices.

Only in the s did the chemical nature of plant of the pectin molecule consist of L -rhamnose, arabinose, tissues become apparent and with this knowledge, sci- galactose and xylose. As a result, pectinases are today one of the partially or completely neutralized by sodium, potassi- upcoming enzymes of the commercial sector. Primarily, um or ammonium ions. Pectinases are Protopectin. This is a parent pectic substance and now an integral part of fruit juice and textile industries upon restricted hydrolysis yields pectin or pectinic acid.

By , the whole world market Kilara, These are the galacturonans that contain Godfrey and West, The main emphasis of this negligible amounts of methoxyl groups. Normal or acid article is on the types of pectinases and their applica- salts of pectic acid are called pectates. Pectinic acids. These are the galacturonans with var- ious amounts of methoxyl groups.

Pectinates are normal or acid salts of pectinic acids Kilara, Kashyap et al. Pectin in native form is located in the sis and whether the cleavage is random endo-, liquefy- cell wall and it may be interlined with other structural ing of depolymerizing enzymes or endwise exo- or polysaccharides and proteins to form insoluble proto- saccharifying enzymes. The three major types of pec- pectin.

Such pectin is insoluble and 3. Pectinesterases PE hence confers rigidity on cell walls. However, during ripening the structure of pectin is altered by naturally Pectinesterases also known as pectinmethyl hydro- occurring enzymes in the fruits. The enzyme acts preferen- chains attached to the units, which make up the main tially on a methyl ester group of galacturonate unit next chain.

Depolymerizing enzymes The pectic substances account for about 0. When the tissue is ground, These are the enzymes: the pectin is found in the liquid phase soluble pectin causing an increase in viscosity and the pulp particles, whereas other pectin molecules remain bound to cellu- 3.

Me- chanical crushing of pectin-rich fruit yields a fruit juice 3. Polymethylgalacturonases PMG. Catalyze the with high viscosity, which remains bound to the pulp in hydrolytic cleavage of a-1,4-glycosidic bonds. Exo-PMG: causes sequential cleavage of a-1,4-glycos- obtained and with higher yields.

In these parti- 3. Polygalacturonases PG. Catalyze hydrolysis of cles, a protein nucleus with a positive surface charge is a-1,4-glycosidic linkages in pectic acid polygalacturonic coated by negatively charged pectin molecules Pilnik acid. They are also of two types: and Voragen, This negative charge causes the 1.

End-PG: also known as poly 1,4-a-D -galacturonide pectin molecules to repel one another. Pectinases de- glycanohydrolase, catalyzes random hydrolysis of a- grade this pectin and expose part of the positively 1,4-glycosidic linkages in pectic acid. Exo-PG: also known as poly 1,4-a-D -galacturonide pulsion between cloud particles which causes these galacturonohydrolase, catalyzes hydrolysis in a se- particles to aggregate to larger particles.

These larger quential fashion of a-1,4-glycosidic linkages on pectic particles eventually settle out, but to improve the pro- acid. Yeasts and 3. What is left is a trans- tion, which results in galacturonide with an unsaturated parent but by no means clear juice. These include: clear juice that many consumers prefer. Polymethylegalacturonate lyases PMGL. Catalyze breakdown of pectin by trans-eliminative 3. They are: 1.

The juices produced by by trans-eliminative cleavage. Polygalacturonate lyases PGL. Catalyze clouds citrus juices, prune juices, tomato juice and cleavage of a-1,4-glycosidic linkage in pectic acid by nectars , and C Unicellular products where the intent trans-elimination. They are also of two types: is to preserve the integrity of the plant cells by selectively 1. Endo-PGL: also known as poly 1,4-a-D -galacturo- hydrolyzing the polysaccharides of the middle lamella.

Exo-PGL: also known as poly 1,4-a-D -galacturonide exolyase, catalyzes sequential cleavage of a-1,4-gly- 4. Sparkling clear juices cosidic linkages in pectic acid. In the case of sparkling clear juices enzymes are added in order to increase the juice yield during pressing 3.

Protopectinase and straining of the juice and to remove suspended matter to give sparkling clear juices free of haze. Some This enzyme solubilizes protopectin forming highly examples of such juices and their further uses are de- polymerized soluble pectin.

On the bases of their applications, pectinases are mainly of two types: acidic pectinases and alkaline 4. Apple juice is manufactured as natural, pectinases. Application of pectinases zymatic treatment Kilara, Acidic pectinases major types of enzymes used in apple juice processing they are by no means the only enzymes used for this Acidic pectic enzymes used in the fruit juice industries purpose.

RK9 PGL NT PG Pectate lyase PAL DT 7 Pectin lyase 8. Glycinea PAL 8. Another potential contributor to the hazi- enzymes. Centrifugation then removes large pieces of ness is starch. This can be broken down using an amylase For a clear juice these suspended particles have to be strictly speaking, an amyloglucosidase active at the pH removed. A dose of commercial enzyme e.

The initial steps in the extraction of cellulases is the accepted way of removing suspended juice from apples include washing, sorting and crushing particles Grassin and Fauquembergue, This en- of apples in a mill.

As these polyphenols in the pulp inhibit pectic Yamaski et al. An alternative PME alone without the presence of contaminating way to remove polyphenols in the fruit juices is the enzymes from the apples. This for the action of pectic enzymes De Vos and Pilnik, research has also shown that apple juice containing Schols et al.

This enzyme was found in Aspergillus aculeatus initially, but it seems also to be produced by other Aspergillus spp. Treatment with pectinase takes anything from 15 min to 2 h depending upon the exact nature of the enzyme and how much is used, the reaction temperature and the variety of apple chosen Kilara, Dur- ing incubation the pectinase degrades soluble pectin in the pulp, which would otherwise hamper juice extraction in two ways.

If a cloudy product is required, the juice is pasteur- ized immediately after pressing, to denature any residual Fig. Production of apple juice Grassin and Fauquembergue, The haze tinases with strong arabanase and arabinosidase activi- may result from polymerization of polyphenols and ties.

The process is similar to that for apples. Pears are oxidation of proanthocyanidins during milling and crushed and enzymes are added to the pulp in macera- pressing. In practice, the total time for processing.

The most important quality factor of these the juice extraction is shorter than the classical process. After mixing Enzymes also help in production of juices and concen- with water, sugar and acid, the cloud particles tend to trates that are very stable and have a good taste with a settle down and sometimes form a gel with a clear su- reduced pomace waste, and reduced production cost pernatant layer: this separation is a serious defect which higher yield, less equipment, less labor in a concentra- reduces the attractiveness of the product Askar et al.

Finally, they also permit the processing of The use of exogenous enzymes can improve the cloud Cider. Traditionally, the fermentation of apple juice was hapect TF Rohm which have high PG and PL activi- very slow a few weeks to 3 months and with some ties combined with cellulases and hemicellulases have residual sugars at the end, depending on the type of ci- been found to decrease the viscosity, keep cloud stabil- der produced dry, semi-sweet and sour.

The cider was ity, and improve the ability to concentrate the product. Pectic enzymes enable the cider pro- and marketed. Grape juice and wine. Grape juice is not con- from the juice. It is mixed with other fruit juices such as apple. Pederson and Luh and Kean have re- 4.





Applications of pectinases in the commercial sector: a review.






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